Position is based on-site, in our Paris BioLabs located in the 14th District

Please submit your CV in English

About the role:

Owkin is currently recruiting a Laboratory Technician to join our Biomedical team. The successful candidate will be a driven, experienced cell/molecular biologist who is enthusiastic about leveraging state-of-the-art techniques and developing translational in vitro complex preclinical assays/models for Owkin’s research objectives, such as evaluating therapeutic targets, identifying biomarkers and delivering high quality data.

The best person for this role is exceptionally rigorous and meticulous in experimental execution, is flexible, and has a demonstrated ability to work in multi-disciplinary collaborations involving a high level of teamwork. This is an exciting opportunity to contribute your in-depth knowledge and technical hand-on skills to our growing BioMedical department and support Owkin's discovery pipeline.

In particular, you will:

• Carry out cell line and patient-derived biobanking, and related experiments (2D and 3D) according to the protocols and the standard operating procedures (SOPs) developed by the team for modeling disease biology.
• Execute general molecular and cellular biology techniques (RNA/DNA work, RT-qPCR, FACS, IHC/IF, IF imaging acquisition for viability/proliferation readouts, high content imaging,...) to generate omics data from in vitro models.
• Execute genetic and drug perturbations experiments to study novel oncology/immuno-oncology targets.
• Carry out assay development and optimisation for the development of disease-relevant complex models and a variety of perturbation readouts.
• Help to coordinate the generation of large datasets with cutting-edge high-throughput spatial transcriptomics methods and ensure consistent practices and SOP compliance throughout partner laboratories.
• Contribute to the SOP writing and updating in collaboration with senior team members.
• Maintain accurate electronic laboratory notebooks in a timely fashion and prepare technical reports, summaries, protocols, etc. Manage and update ELN inventories and databases.
• Report experimental results orally to the Team, Program leads and BioMedical department in regular meetings.
• Document deviations from the protocol / experimental plan and operating methods and inform the Lab Director.
• Be responsible for monitoring the maintenance of equipment.
• Train lab newcomers as required and contribute to building a cohesive and highly performing experimental team.

About you

Education & Experience:

• Bachelor of Science or equivalent (BTS, DUT, Licence) in molecular/cellular biology, biochemistry, biotechnology or related disciplines. MSc is a plus.
• Minimum of 5 years experience working ideally in an industrial setting, with the following technical and scientific capabilities:
  • Isolating and handling donor-derived cells and performing cell culture and flow cytometry based assays (apoptosis assays, cell cycle determination and multicolor staining).
  • Classical molecular biology techniques (cloning, PCR, RT-qPCR, Western Blot) and vector creation (CRISPR Cas-9/RNAi).
  • Immunology such as PBMC purification and immuno-assays on immune and co-culture assays.
  • 3D model systems (matrix-attached models, spheroids and/or organoids) using primary cells is a plus.
  • Plate-based functional assays such as cell viability assays (e.g. CTG), apoptosis assays (e.g. Caspase), etc.
  • Imaging 2D and/or 3D cultures and acquiring fluorescence and bright field microscopy images is a plus.
  • Histology and immunohistology techniques are a plus.
  • A practical understanding of assay optimisation to enable generation of reproducible cellular assays.

Skills & Competencies:

• General understanding of cancer biology and related immunology, coupled with comprehension of the crucial steps of target validation.
• Curiosity: You are driven to understand how components fit together within a complex system.
• Proactively scouts for and evaluates technical developments in the field of disease tumor models.
• Excellent communication skills, with the ability to present research succinctly to diverse audiences.
• Proven ability to work both independently and collaboratively within a fast-paced, team-oriented environment.
• Adept at independently troubleshooting well-established experiments.
• Exceptional attention to detail and meticulous record-keeping skills.
• Capable of interacting effectively across diverse interfaces of discipline, culture, and expertise.
• Fluency in English is mandatory.

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